He also starred as Justin in Cybill, Jimmy in Roseanne, and has made several guest appearances on Punk'd and MADtv. His younger brother is actor Christopher Masterson of the television series Malcolm in the Middle.

He is a co-owner of Dolce, a restaurant he founded with That '70s Show co-stars Ashton Kutcher and Wilmer Valderrama. Masterson also frequently DJs at Los Angeles night clubs under his name, DJ Donkey Punch. Masterson also co-hosts an extremely popular radio show titled "Feel My Heat", on Indie 103.1 in Los Angeles. As co-host of this show, he frequently displays a knack for a Howard Stern-esque sense of humor, as exemplified by a dialogue he once initiated concerning a canine's sex organs. He started out by musing on what a dog's vagina is officially referred to as, then continued for ten minutes longer, quizzing everyone in his studio as to what the official name for a dog's vagina could possibly be (despite the fact that everyone already knew there is no special term). He is currently dating actress Bijou Phillips.

Danny Masterson is a member of the Church of Scientology, as his parents raised him in the beliefs of Scientology. In December 2005 he helped promote the gala opening of Scientology's controversial "Psychiatry: An Industry of Death" Museum, which puts forth a conspiracy theory connecting Adolf Hitler to the psychiatric profession.

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