Jack McBrayer was born in Macon, Georgia, and moved to Conyers, Georgia when he was 15. McBrayer studied theater administration at the University of Evansville. He later worked at The Second City and IO Theater (1995–2002), an experience that introduced him to 30 Rock creator Tina Fey and prepared him "100%" for his role on her show.

McBrayer appeared in over 80 sketches on Late Night with Conan O'Brien (2002–04), often lampooning his own Southern upbringing by playing stereotypical hillbilly characters. On August 10, 2007 and September 20, 2008, he made a cameo appearance on Late Night playing Kenneth Parcell from 30 Rock. McBrayer later reprised his role as Kenneth twice (November 24, 2009 and January 13, 2010) after O'Brien's switch to The Tonight Show, and has appeared on Conan's Legally Prohibited From Being Funny on Television Tour at stops in Eugene, Oregon, Universal City, California (on the Universal Studios lot where O'Brien taped Tonight), and the final tour stop in Atlanta, in his home state of Georgia.

McBrayer also had a minor recurring role as a waiter at the Bluth family's country club in Arrested Development. He works as a voice actor in the American animated series Ugly Americans and appeared in the 2010 film Cats & Dogs: The Revenge of Kitty Galore with a recurring role on the third season of Phineas and Ferb, playing the part of Irving, a fan of Phineas and Ferb. He also regularly appears in the skit "Knock Knock Joke of the Day" on the hit children's television show, Yo Gabba Gabba! since season 2 and stars in Craig McCracken's new TV show Wander Over Yonder on the Disney Channel. In the fall of 2012, Jack lent his voice to the Disney animated film Wreck-It Ralph, playing the title character's video game opponent 'Fix-It Felix, Jr.'.

Television Personalities,